Welcome to Dr. Kate Brilakis' Learning Portal

          one bp
              =
       base pair

we're using bacteriophage lambda DNA

  step 2:
isolating DNA sample

we will use three REs:
PstI
EcoRI
HindIII

     DNA Analysis

    let's review
        DNA...

 step 1:
‚Äč collecting DNA sample

One Nucleotide

  step 3:
 cutting DNA sample into fragments
 
using restriction endonuclease enzymes

different restriction enzyme recognize and cleaves different palindromic restriction sites

  step 6:
 analyzing DNA fragments

  step 4:
 separation of DNA fragments using gel electrophoresis

  step 5:
 staining of DNA fragments
 separated 
using gel electrophoresis

Analyzing DNA using a     DNA "Fingerprint"